We obtained 67,539 barcoded reads (size range, 51 to 91,079 nucleotides [nt]; N 50, 12,320 nt). Demultiplexed raw subreads were downloaded from PacBio SMRT Link, converted to .fastq files with bam2fastx version 1.3.1, and then chimera checked, quality controlled, assembled, and circularized using Flye version 2.8.2 ( 8 ).
